期刊
STEM CELL REPORTS
卷 8, 期 3, 页码 715-727出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2017.01.008
关键词
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资金
- John S. Dunn Foundation (P.W.)
- Brown Foundation of Houston
- NIH [R24AI120942, R21AI12950901, 4T32DA007287]
- Chief Research Office at the University of Texas Medical Branch
Zika virus (ZIKV) infection causes microcephaly in a subset of infants born to infected pregnant mothers. It is unknown whether human individual differences contribute to differential susceptibility of ZIKV-related neuropathology. Here, we use an Asian-lineage ZIKV strain, isolated from the 2015 Mexican outbreak (Mex1-7), to infect primary human neural stem cells (hNSCs) originally derived from three individual fetal brains. All three strains of hNSCs exhibited similar rates of Mex1-7 infection and reduced proliferation. However, Mex1-7 decreased neuronal differentiation in only two of the three stem cell strains. Correspondingly, ZIKA-mediated transcriptome alterations were similar in these two strains but significantly different from that of the third strain with no ZIKV-induced neuronal reduction. This study thus confirms that an Asian-lineage ZIKV strain infects primary hNSCs and demonstrates a cell-strain-dependent response of hNSCs to ZIKV infection.
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