期刊
STEM CELL REPORTS
卷 9, 期 1, 页码 397-407出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2017.05.026
关键词
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资金
- Reproductive Scientist Development Program (NIH) [5K12HD000849]
- American Society for Reproductive Medicine
- Howard and Georgeanna Jones Foundation
- Einstein Nathan Shock Center
- NIH [P01AG017242]
- Glenn Foundation
- SENS Foundation
The establishment of DNA methylation patterns in oocytes is a highly dynamic process marking gene-regulatory events during fertilization, embryonic development, and adulthood. However, after epigenetic reprogramming in primordial germ cells, how and when DNA methylation is re-established in developing human oocytes remains to be characterized. Here, using single-cell whole-genome bisulfite sequencing, we describe DNA methylation patterns in three different maturation stages of human oocytes. We found that while broad-scale patterns of CpG methylation have been largely established by the immature germinal vesicle stage, localized changes continue into later development. Non-CpG methylation, on the other hand, undergoes a large-scale, generalized remodeling through the final stage of maturation, with the net overall result being the accumulation of methylation as oocytes mature. The role of the genome-wide, non-CpG methylation remodeling in the final stage of oocyte maturation deserves further investigation.
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