期刊
STEM CELL REPORTS
卷 8, 期 4, 页码 803-812出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2017.02.016
关键词
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资金
- Wellcome Trust
- MRC
- German Research Foundation [DFG PA2369/1-1]
- British Heart Foundation PhD Studentship [FS/11/77/39327]
- Clinician Scientist Award from the National Institute for Health Research UK [CS-2015-15-023]
- Qatar Foundation
- National Institutes of Health Research (NIHR) [CS-2015-15-023] Funding Source: National Institutes of Health Research (NIHR)
- British Heart Foundation [FS/10/022/28262] Funding Source: researchfish
- Medical Research Council [G0800784, MC_PC_12009, G1000847, G0600275] Funding Source: researchfish
- National Institute for Health Research [CS-2015-15-023] Funding Source: researchfish
- MRC [G0600275, G0800784, G1000847] Funding Source: UKRI
The isolation or in vitro derivation of many human cell types remains challenging and inefficient. Direct conversion of human pluripotent stem cells (hPSCs) by forced expression of transcription factors provides a potential alternative. However, deficient inducible gene expression in hPSCs has compromised efficiencies of forward programming approaches. We have systematically optimized inducible gene expression in hPSCs using a dual genomic safe harbor gene-targeting strategy. This approach provides a powerful platform for the generation of human cell types by forward programming. We report robust and deterministic reprogramming of hPSCs into neurons and functional skeletal myocytes. Finally, we present a forward programming strategy for rapid and highly efficient generation of human oligodendrocytes.
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