4.6 Article

Replicable Expansion and Differentiation of Neural Precursors from Adult Canine Skin

期刊

STEM CELL REPORTS
卷 9, 期 2, 页码 557-570

出版社

CELL PRESS
DOI: 10.1016/j.stemcr.2017.07.008

关键词

-

资金

  1. Rebecca L. Cooper Medical Research Foundation
  2. ANZ Mason Foundation
  3. National Health and Medical Research Council (NHMRC) of Australia Program Grant [568969]

向作者/读者索取更多资源

Repopulation of brain circuits by neural precursors is a potential therapeutic strategy for neurodegenerative disorders; however, choice of cell is critical. Previously, we introduced a two-step culture system that generates a high yield of neural precursors from small samples of adult canine skin. Here, we probe their gene and protein expression profiles in comparison with dermal fibroblasts and brain-derived neural stem cells and characterize their neuronal potential. To date, we have produced >50 skin-derived neural precursor (SKN) lines. SKNs can be cultured in a highly replicable fashion and uniformly express a panel of identifying markers. Upon differentiation, they self-upregulate neural specification genes, generating neurons with basic electrophysiological functionality. This unique population of neural precursors, derived from mature skin, overcomes many of the practical issues that have limited clinical translation of alternative cell types. Easily accessible, neuronally committed, and patient specific, SKNs may have potential for the treatment of brain disorders.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据