期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 124, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/55704
关键词
Medicine; Issue 124; Murine cochlear explant; organotypic culture; inner ear; outer hair cells; inner hair cells; spiral ganglion neurons; auditory synaptopathy; adeno-associated virus
资金
- National Institute of Deafness and Other Communication Disorders [R01DC015824, T32DC00038]
- Department of Defense [W81XWH-15-1-0472]
- Bertarelli Foundation
- Nancy Sayles Day Foundation
- Lauer Tinnitus Research Center
While there have been remarkable advances in hearing research over the past few decades, there is still no cure for Sensorineural Hearing Loss (SNHL), a condition that typically involves damage to or loss of the delicate mechanosensory structures of the inner ear. Sophisticated in vitro and ex vivo assays have emerged in recent years, enabling the screening of an increasing number of potentially therapeutic compounds while minimizing resources and accelerating efforts to develop cures for SNHL. Though homogenous cultures of certain cell types continue to play an important role in current research, many scientists now rely on more complex organotypic cultures of murine inner ears, also known as cochlear explants. The preservation of organized cellular structures within the inner ear facilitates the in situ evaluation of various components of the cochlear infrastructure, including inner and outer hair cells, spiral ganglion neurons, neurites, and supporting cells. Here we present the preparation, culture, treatment, and immunostaining of neonatal murine cochlear explants. The careful preparation of these explants facilitates the identification of mechanisms that contribute to SNHL and constitutes a valuable tool for the hearing research community.
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