期刊
ACS OMEGA
卷 1, 期 2, 页码 193-201出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.6b00119
关键词
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资金
- JSPS KAKENHI [2408, JP24109008]
- Grants-in-Aid for Scientific Research [26221001, 24109008, 26410121] Funding Source: KAKEN
Among biologically active compounds, calcium ions (Ca2+) are one of the most important species in cell physiological functions. Development of new calcium chelators with two-photon absorption (TPA) properties is a state-of-the-art challenge for chemists. In this study, we report the first and efficient synthesis of 5-bromo-2-nitrobenzyl-substituted ethylene glycol tetraacetic acid (EGTA) as a platform for a new generation of calcium chelators with TPA properties in the near-infrared region. New calcium chelators with high TPA properties, that is, a two-photon (TP) fragmentation efficiency of delta(u) = 20.7 GM at 740 nm for 2-(4-nitrophenyl) benzofuran (NPBF)-substituted EGTA (NPBF-EGTA, K-d = 272 nM) and delta(u) = 7.8 GM at 800 nm for 4-amino-4'-nitro-1,1'-biphenyl (BP)-substituted EGTA (BP-EGTA, K-d = 440 nM) derivatives, were synthesized using Suzuki-Miyaura coupling reactions of the bromide with benzofuran-2-boronic acid and 4-(dimethylamino) phenyl boronic acid, respectively. The corresponding acetoxymethyl (AM) esters were prepared and successfully applied to the Ca2+-uncaging reaction triggered by TP photolysis in vivo.
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