期刊
ENEURO
卷 3, 期 2, 页码 -出版社
SOC NEUROSCIENCE
DOI: 10.1523/ENEURO.0078-16.2016
关键词
AMPA receptors; cochlear afferents; outer hair cells; postsynaptic densities
资金
- National Institute on Deafness and Other Communication Disorders (NIDCD) [R01-DC-006476, R01-DC-011741, P30-DC-005211, T32-DC-000023, F31-DC-010948]
- Multiphoton Imaging Core grant [NS050274]
- John Mitchell, Jr. Trust
- David M. Rubenstein Fund for Hearing Research
- Intramural Research Program of the National Institutes of Health, NIDCD
Mechanosensory hair cells release glutamate at ribbon synapses to excite postsynaptic afferent neurons, via AMPA-type ionotropic glutamate receptors (AMPARs). However, type II afferent neurons contacting outer hair cells in the mammalian cochlea were thought to differ in this respect, failing to show GluA immunolabeling and with many ribbonless afferent contacts. Here it is shown that antibodies to the AMPAR subunit GluA2 labeled afferent contacts below inner and outer hair cells in the rat cochlea, and that synaptic currents in type II afferents had AMPAR-specific pharmacology. Only half the postsynaptic densities of type II afferents that labeled for PSD-95, Shank, or Homer were associated with GluA2 immunopuncta or presynaptic ribbons, the empty slots corresponding to ribbonless contacts described previously. These results extend the universality of AMPAergic transmission by hair cells, and support the existence of silent afferent contacts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据