期刊
ENEURO
卷 3, 期 6, 页码 -出版社
SOC NEUROSCIENCE
DOI: 10.1523/ENEURO.0358-16.2016
关键词
axon regeneration; conditioning lesion; dorsal root ganglion; mTOR; spinal cord injury; Stat3
资金
- Research Grants Council, University Grants Committee (RGC, UGC) [T13-607/12R, 689913, 16101414, 16103315]
- Ministry of Science and Technology of the People's Republic of China (MOST) [2013CB530900]
- FHB \ Health and Medical Research Fund (HMRF) [02133016]
- National Natural Science Foundation of China (NSFC) [81671214, 81272773]
Neuronal mammalian target of rapamycin (mTOR) activity is a critical determinant of the intrinsic regenerative ability of mature neurons in the adult central nervous system (CNS). However, whether its action also applies to peripheral nervous system (PNS) neurons after injury remains elusive. To address this issue unambiguously, we used genetic approaches to determine the role of mTOR signaling in sensory axon regeneration in mice. We showed that deleting mTOR in dorsal root ganglion (DRG) neurons suppressed the axon regeneration induced by conditioning lesions. To establish whether the impact of mTOR on axon regeneration results from functions of mTOR complex 1 (mTORC1) or 2 (mTORC2), two distinct kinase complexes, we ablated either Raptor or Rictor in DRG neurons. We found that suppressing mTORC1 signaling dramatically decreased the conditioning lesion effect. In addition, an injury to the peripheral branch boostsmTORactivity inDRGneurons that cannot be completely inhibited by rapamycin, a widely used mTOR-specific inhibitor. Unexpectedly, examining several conditioning lesion-induced pro-regenerative pathways revealed that Raptor deletion but not rapamycin suppressed Stat3 activity in neurons. Therefore, our results demonstrate that crosstalk between mTOR and Stat3 signaling mediates the conditioning lesion effect and provide genetic evidence that rapamycin-resistant mTOR activity contributes to the intrinsic axon growth capacity in adult sensory neurons after injury.
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