期刊
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
卷 37, 期 3, 页码 137-148出版社
SPRINGER INT PUBL AG
DOI: 10.1017/S1742758417000133
关键词
Apis mellifera jemenitica; molecular detection; Paenibacillus larvae; Saudi Arabia
类别
资金
- National Plan for Science, Technology and Innovation (MAARIFAH)
- King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia [11-AGR2082-02]
Apis mellifera jemenitica, the only indigenous honey bee race of Saudi Arabia, is well adapted to the harsh local environmental conditions. A large-scale field survey was conducted to screen major Saudi Arabian beekeeping locations for infection by Paenibacillus larvae. Paenibacillus larvae is one of the major bacterial pathogens of honey bee broods and is the causative agent of American foulbrood disease. Larvae from samples suspected of infection were collected from different apiaries and homogenized in phosphate-buffered saline. Bacteria were isolated on MYPGP agar medium. Two bacterial isolates, ksuPL3 and ksuPL5 (16S rRNA GenBank accession numbers, KR780760 and KR780761, respectively), were subjected to molecular identification using P. larvae-specific primers. A BLAST sequence analysis revealed that the two isolates were P. larvae with more than 98% sequence identity. This detection of P. larvae in the indigenous honey bee is the first recorded incidence of this pathogen in Saudi Arabia. This study emphasizes the need for the relevant authorities to take immediate steps towards treating and limiting the spread of this disease throughout the country.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据