期刊
CANCER MEDICINE
卷 7, 期 1, 页码 146-156出版社
WILEY
DOI: 10.1002/cam4.1237
关键词
EMT; gastric cancer; miR-192/-215; SMG-1; Wnt signaling pathway
类别
资金
- National Nature Science Foundation of China [81772592, 31601028]
- National Natural Youth Science Foundation of China [81302151]
- Shenzhen Peacock Plan [KQCX20130621101141669]
- Planned Science and Technology Project of Shenzhen [JCYJ20140418095735574, JCYJ20160422170722474]
- Key Laboratory Project of Shenzhen [ZDSY20130329101130496]
- Medical Science and Technology Research Foundation of Guangdong Province [A2016112]
- Science and Technology Bureau of Shenzhen City [JCYJ20150525092940973]
- NIH [DK087454, CA146799, CA173390]
- American Cancer Society Clinical Research Professorship
SMG-1,a member of the phosphoinositide kinase-like kinase family, functioned as a tumor suppressor gene. However, the role of SMG-1 in GC remain uncharacterized. In this study, regulation of SMG-1 by miR-192 and-215, along with the biological effects of this modulation, were studied in GC. We used gene microarrays to screening and luciferase reporter assays were to verify the potential targets of miR-192 and-215. Tissue microarrays analyses were applied to measure the levels of SMG-1 in GC tissues. Western blot assays were used to assess the signaling pathway of SMG-1 regulated by miR-192 and-215 in GC. SMG-1 was significantly downregulated in GC tissues.The proliferative and invasive properties of GC cells were decreased by inhibition of miR-192 and-215, whereas an SMG-1siRNA rescued the inhibitory effects. Finally, SMG-1 inhibition by miR-192 and-215 primed Wnt signaling and induced EMT. Wnt signaling pathway proteins were decreased markedly by inhibitors of miR-192 and-215, while SMG-1 siRNA reversed the inhibition apparently. Meanwhile, miR-192 and-215 inhitibtors increased E-cadherin expression and decreased N-cadherin and cotransfection of SMG-1 siRNA reversed these effects. In summary, these findings illustrate that SMG-1 is suppressed by miR-192 and-215 and functions as a tumor suppressor in GC by inactivating Wnt signaling and suppressing EMT.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据