期刊
BIOLOGY OPEN
卷 6, 期 3, 页码 317-325出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/bio.023887
关键词
Notch; Pericyte; Neural crest; Frontonasal mesenchyme; Olfactory ensheathing cells; Chick embryo
类别
资金
- Anatomical Society
- Cambridge Philosophical Society
- Wellcome Trust [102453/Z/13/Z]
- Cambridge Commonwealth Trust
- Wellcome Trust [102453/Z/13/Z] Funding Source: Wellcome Trust
Perivascular/mural cells originate from either the mesoderm or the cranial neural crest. Regardless of their origin, Notch signalling is necessary for their formation. Furthermore, in both chicken and mouse, constitutive Notch1 activation (via expression of the Notch1 intracellular domain) is sufficient in vivo to convert trunk mesoderm-derived somite cells to perivascular cells, at the expense of skeletal muscle. In experiments originally designed to investigate the effect of premature Notch1 activation on the development of neural crest-derived olfactory ensheathingglial cells (OECs), we used in ovo electroporation to insert a tetracycline-inducible Notch Delta E construct (encoding a constitutively active mutant of mouse Notch1) into the genome of chicken cranial neural crest cell precursors, and activated Notch Delta E expression by doxycycline injection at embryonic day 4. Notch Delta E-targeted cells formed perivascular cells within the frontonasal mesenchyme, and expressed a perivascular marker on the olfactory nerve. Hence, constitutively activating Notch1 is sufficient in vivo to drive not only somite cells, but alsoneural crest-derived frontonasal mesenchyme and perhaps developing OECs, to a perivascular cell fate. These results also highlight the plasticity of neural crest-derived mesenchyme and glia.
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