期刊
BIOLOGY OPEN
卷 6, 期 2, 页码 252-259出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/bio.021774
关键词
MiR-142; Pancreatic cancer; Cell proliferation; Cell invasion; HIF-1 alpha; Hypoxia microenvironment
类别
MicroRNAs regulate most protein-coding genes, including genes important in cancer and other diseases. In this study, we demonstrated that the expression of miR-142 could be significantly suppressed in pancreatic cancer specimens and cell lines compared to their adjacent tissues and normal pancreatic cells. Growth and invasion of PANC-1 and SW1990 cells were attenuated by overexpression of miR-142 in vitro. With the help of bioinformatics analysis, hypoxia-inducible factor 1 (HIF-1 alpha) was identified to be a direct target of miR-142, and a luciferase reporter experiment confirmed this discovery. Overexpression of miR-142 decreases protein expression of HIF-1 alpha. In the hypoxic microenvironment, HIF-1 alpha was up-regulated while miR142 was down-regulated. The invaded cells significantly increased in the hypoxic microenvironment compared to the normoxic microenvironment. The hypoxia treatment induced cells' proliferation, and invasion could be inhibited by miR-142 overexpression or HIF-1 alpha inhibition. Moreover, expression of epithelial-mesenchymal transition (EMT) markers, Vimentin, VEGF-C and E-cad, was altered under hypoxia conditions and regulated by miR-142/HIF-1 alpha. Above all, these findings provided insights on the functional mechanism of miR-142, suggesting that the miR-142/HIF-1 alpha axis may interfere with the proliferative and invasive properties of pancreatic cancer cells, and indicated that miR-142 could be a potential therapeutic target for pancreatic cancer.
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