期刊
APPLICATIONS IN PLANT SCIENCES
卷 5, 期 8, 页码 -出版社
BOTANICAL SOC AMER INC
DOI: 10.3732/apps.1700010
关键词
Chinese traditional medicine; conservation; Loranthaceae; microsatellite marker; next-generation sequencing; Taxillus nigrans; transcriptome
资金
- National Key Research and Development Program of China [2016YFD0600203]
- National Natural Science Foundation of China [31622015, 31200477]
- Department of Science and Technology of Sichuan Province [2013JY0026, 2015JQ0018]
Premise of the study: We developed transcriptome microsatellite markers (simple sequence repeats) for Taxillus nigrans (Loranthaceae) to survey the genetic diversity and population structure of this species. Methods and Results: We used Illumina HiSeq data to reconstruct the transcriptome of T. nigrans by de novo assembly and used the transcriptome to develop a set of simple sequence repeat markers. Overall, 40 primer pairs were designed and tested; 19 of them amplified successfully and demonstrated polymorphisms. Two loci that detected null alleles were eliminated, and the remaining 17, which were subjected to further analyses, yielded two to 21 alleles per locus. Conclusions: The markers will serve as a basis for studies to assess the extent and pattern of distribution of genetic variation in T. nigrans, and they may also be useful in conservation genetic, ecological, and evolutionary studies of the genus Taxillus, a group of plant species of importance in Chinese traditional medicine.
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