期刊
APPLICATIONS IN PLANT SCIENCES
卷 5, 期 5, 页码 -出版社
BOTANICAL SOC AMER INC
DOI: 10.3732/apps.1700035
关键词
Calydorea crocoides; Herbertia darwinii; Herbertia zebrina; Illumina MiSeq; Iridaceae; next-generation sequencing; simple sequence repeat (SSR) marker
资金
- Deutscher Akademischer Austauschdienst (DAAD)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [304197/2012-2]
- German Research Foundation [KO1741/3-1]
- Fundacao Grupo Boticario de Protecao a Natureza [1018/20142]
Premise of the study: Polymorphic microsatellite loci were developed and used to genotype individuals of Herbertia zebrina (Iridaceae) as a first step for assessment of intraspecific genetic diversity. Methods and Results: Primer pairs for 47 markers were developed: 20 from a microsatellite-enriched library and 27 from a next-generation sequencing run using the Illumina MiSeq platform. Of those, 15 loci were considered successful, of which 12 were polymorphic and three were monomorphic. The primers were tested in 50 individuals from three populations of H. zebrina. Two to 14 alleles per locus were identified, and observed and expected heterozygosity were 0.00-0.95 and 0.18-0.89, respectively. Tests of cross-amplification to evaluate the applicability of these markers showed positive results in one congeneric species, H. darwinii, and in a phylogenetically closely related species, Calydorea crocoides. Conclusions: These microsatellite markers can be used for studies of genetic variation and genetic population structure, as well as to support conservation efforts.
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