期刊
ACS PHOTONICS
卷 4, 期 2, 页码 211-216出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsphotonics.6b00912
关键词
single-molecule imaging; label free; interferometric scattering; biosensing
类别
资金
- ERC [337577]
- EPSRC [EP/M025241/1]
- Zvi and Ofra Meitar Magdalen Graduate Scholarship
- EPSRC [EP/M002144/1, EP/M025241/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/M002144/1, EP/M025241/1] Funding Source: researchfish
Our ability to optically interrogate nanoscopic objects is controlled by the difference between their extinction cross sections and the diffraction-limited area to which light can be confined in the far field. We show that a partially transmissive spatial mask placed near the back focal plane of a high numerical aperture microscope objective enhances the extinction contrast of a scatterer near an interface by approximately T-1/2, where T is the transmissivity of the mask. Numerical-aperture-based differentiation of background from scattered light represents a general approach to increasing extinction contrast and enables routine label-free imaging down to the single-molecule level.
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