4.1 Article

Adipose-derived mesenchymal stem cells suppress of acute rejection in small bowel transplantation

期刊

SAUDI JOURNAL OF GASTROENTEROLOGY
卷 23, 期 6, 页码 223-229

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MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/sjg.SJG_122_17

关键词

Acute rejection; adipose-derived mesenchymal stem cells; small bowel transplantation

资金

  1. National Natural Science Foundation of People's Republic of China [81470982]
  2. Tianjin Municipal Health Bureau Key Project of People's Republic of China [16KG105]

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Background/Aims: Adipose-derived mesenchymal stem cells (ADSCs) possess immunosuppressive activity and hold promise in autologous cell-based therapies. The aim of this study was to determine whether autologous ADSCs can improve outcomes in the rat small bowel transplantation (SBT) model. Materials and Methods: Allogeneic SBT followed by implantation of autologous ADSCs through the penile vein was conducted in Brown-Norway (BN) donor rats with Lewis (LEW) recipient rats infused with phosphate buffered solution as the control. Allograft and recipient peripheral blood were obtained. We assessed histopathology, apoptosis, cytokines, and regulatory T cells (Tregs). One-way analysis of variance was applied to assess the significance of the data. Results: It was found that ADSCs significantly reduced acute rejection and improved the allograft's survival rate. In addition, there were significantly fewer apoptotic cells in allograft mucosae in the ADSC group in comparison with the control group. Furthermore, levels of interleukin (IL)-10 and transforming growth factor-beta 1 were significantly elevated, whereas those of IL-2 and IL-17 levels were significantly reduced in the ADSC group when compared to the control group. Moreover, flow cytometry analysis revealed that there were significantly more peripheral Tregs after the infusion of ADSCs. Conclusions: These results demonstrate that implanted autologous ADSCs improve allogeneic small bowel allograft outcomes by attenuating the acute rejection and reducing inflammatory responses.

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