期刊
LIGHT-SCIENCE & APPLICATIONS
卷 7, 期 -, 页码 -出版社
CHINESE ACAD SCIENCES, CHANGCHUN INST OPTICS FINE MECHANICS AND PHYSICS
DOI: 10.1038/lsa.2017.153
关键词
cranial window; dendritic spines; optical clearing; synaptic resolution; two-photon imaging
类别
资金
- National Natural Science Foundation of China [91232710, 31571002]
- Science Fund for Creative Research Groups [61721092]
- Director Fund of WNLO
Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here, we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy, this technique allowed us to repeatedly image neurons, microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation. Given its easy handling and safety, this method holds great promise for application in neuroscience research.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据