期刊
JOURNAL OF WOUND CARE
卷 26, 期 7, 页码 S24-S33出版社
MA HEALTHCARE LTD
DOI: 10.12968/jowc.2017.26.Sup7.S24
关键词
wound healing; biofilm; infection; Pseudomonas aeruginosa; cyclic di-GMP
类别
资金
- American Diabetes Association [7-13-BS-180]
Objective: To examine how bacterial biofilms, as contributing factors in the delayed closure of chronic wounds in patients with diabetes, affect the healing process. Method: We used daily microscopic imaging and the IVIS Spectrum in vivo imaging system to monitor biofilm infections of bioluminescent Pseudomonas aeruginosa and evaluate healing in non-diabetic and streptozotocin-induced diabetic mice. Results: Our studies determined that diabetes alone did not affect the rate of healing of full-depth murine back wounds compared with nondiabetic mice. The application of mature biofilms to the wounds significantly decreased the rate of healing compared with non-infected wounds for both non-diabetic as well as diabetic mice. Diabetic mice were also more severely affected by biofilms displaying elevated pus production, higher mortality rates and statistically significant increase in wound depth, granulation/fibrosis and biofilm presence. Introduction of a mutant Pseudomonas aeruginosa capable of producing high concentrations of cyclic di-GMP did not result in increased persistence in either diabetic or non-diabetic animals compared with the wild type strain. Conclusion: Understanding the interplay between diabetes and biofilms may lead to novel treatments and better clinical management of chronic wounds.
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