期刊
CHEMICAL ENGINEERING JOURNAL
卷 307, 期 -, 页码 939-947出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2016.09.006
关键词
Cashew apple bagasse; SSF; Fed-batch SSF; Second generation ethanol
资金
- ANP
- CAPES
- CNPq (Federal)
Simultaneous saccharification and fermentation (SSF) is a promising process for the bioconversion of lignocellulosic biomass. Furthermore, an efficient approach to reduce the capital costs for the production of bio-based products is the use of high glucan loading. Therefore, in this study, a comparison of SSF strategies were investigated aiming to enhance ethanol production from acidic-alkaline pretreated cashew apple bagasse (CAB-OH) by Kluyveromyces marxianus ATCC36907 at high glucan loading. An ethanol concentration of 58 g/L was achieved with 15% CAB-OH using batch SSF, resulting in an 81.2% overall ethanol yield. Prehydrolysis of 12 h, prior to SSF, did not significantly increase the overall ethanol yield. Fed-batch SSF, using high loadings of solids, was also investigated. Ethanol concentrations of up to 67 g/L could be produced from CAB-OH (20% w/v) by adding fresh substrate every 4 h during the first 48 h of SSF (10% initial and 2.5% of feeding), achieving an overall ethanol yield of 81%. In the fed-batch mode, the amount of enzyme was lower than used in batch and this process allowed higher ethanol concentrations and similar yield. No major differences in fed-batch performance, considering ethanol concentration and yield, were observed for the different feeding amounts, around 68 g/L and 80.7%, respectively. (C) 2016 Elsevier B.V. All rights reserved.
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