期刊
ANALYTICAL BIOCHEMISTRY
卷 516, 期 -, 页码 65-74出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2016.10.016
关键词
Intein tag; High throughput protein purification; Self-cleaving tag; Elastin-like polypeptide; Affinity methods; Recombinant protein purification
资金
- National Science Foundation [1264322]
- US Army Research Office [W911NF-11-1-0118]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1264322] Funding Source: National Science Foundation
High throughput methods for recombinant protein production using E. coli typically involve the use of affinity tags for simple purification of the protein of interest. One drawback of these techniques is the occasional need for tag removal before study, which can be hard to predict In this work, we demonstrate two high throughput purification methods for untagged protein targets based on simple and cost-effective self-cleaving intein tags. Two model proteins, E. coli beta-galactosidase (beta Gal) and super folder green fluorescent protein (sfGFP), were purified using self-cleaving versions of the conventional chitin-binding domain (CBD) affinity tag and the nonchromatographic elastin-like-polypeptide (ELP) precipitation tag in a 96-well filter plate format. Initial tests with shake flask cultures confirmed that the intein purification scheme could be scaled down, with >90% pure product generated in a single step using both methods. The scheme was then validated in a high throughput expression platform using 24 well plate cultures followed by purification in 96-well plates. For both tags and with both target proteins, the purified product was consistently obtained in a single-step, with low well-to-well and plate-to-plate variability. This simple method thus allows the reproducible production of highly pure untagged recombinant proteins in a convenient microtiter plate format. (C) 2016 Elsevier Inc. All rights reserved.
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