期刊
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
卷 55, 期 1, 页码 123-131出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/cclm-2016-0205
关键词
acute myeloid leukemia; CDH1 (E-cadherin); expression; prognosis
资金
- National Natural Science foundation of China [81270630, 81172592]
- Science and Technology Special Project in Clinical Medicine of Jiangsu Province [BL2012056]
- 333 Project of Jiangsu Province [BRA2013136]
- Six talent peaks project in Jiangsu Province [2015-WSN-115]
- Science and Technology Infrastructure Program of Zhenjiang [SS2012003]
- Social Development Foundation of Zhenjiang [SH2014086, SH2014044, SH2015058]
- Clinical Medical Science, Development Foundation of Jiangsu University [JLY20140018]
- Key Medical Talent Program of Zhenjiang City
Background: Epithelial-mesenchymal transition (EMT) is a critical process which involves in tumor metastasis. As an important EMT marker gene, CDH1 (E-cadherin) expression and its clinical implication in acute myeloid leukemia (AML) remain largely elusive. Methods: Real-time quantitative PCR (RQ-PCR) was carried out to examine CDH1 transcript level in 123 de novo AML patients and 34 controls. Results: Compared with controls, CDH1 was significantly downregulated in AML (p < 0.001). The median level of CDH1 expression divided total AML patients into CDH1 low-expressed (CDH1(low)) and CDH1 high-expressed (CDH1(high)) groups. There were no significant differences between the two groups in age, peripheral blood cell counts, complete remission (CR) rate, and the distribution of FAB/WHO subtypes as well as karyotypes/karyotypic classifications (p > 0.05). However, CDH1(low) group tended to have a higher bone marrow (BM) blasts (p = 0.093). The spearman correlation analysis further illustrated a trend towards a negative correlation between CDH1 expression level and BM blasts (r =-0.214, p = 0.052). CDH1(low) group had a tendency towards a lower frequency of N/K-RAS mutations (p = 0.094). Furthermore, CDH1(low) patients had markedly shorter overall survival (OS) time in cytogenetic normal AML (CN-AML) (p = 0.019). Both univariate and multivariate analyses confirmed the prognostic value of CDH1 expression in CN-AML patients (p = 0.027 and 0.033, respectively). Conclusions: CDH1 downregulation acted as an independent prognostic biomarker in CN-AML patients.
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