期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 5, 期 6, 页码 1246-1257出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6tb02959k
关键词
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资金
- Hi-Tech Research and Development Program of China (863 Program) [2012AA020502]
- National Key Basic Research Program of China (973 programs) [2014CB542202]
- National Natural Science Foundation of China [81130080, 81402447, 81471255, 31300942]
- Basic Research Program of Jiangsu Education Department [14KJA310004]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
Extracellular/acellular matrix-containing neural scaffolds represent a promising design of a tissue engineered nerve graft (TENG) for peripheral nerve repair. In this study, we engineered a composite neural scaffold by culturing dog bone marrow mesenchymal stem cells (BMSCs) onto the surface of a chitosan/silk fibroin-based scaffold and then exposing the cell culture to decellularization to deposit acellular matrix (ACM) coatings on the scaffold. This natural biomaterial-based, cell-derived ACM-coated neural scaffold, as a novel nerve graft, was used to bridge a 60 mm long nerve gap in a dog sciatic nerve. At 12 months after grafting, behavioral, functional, and histological evaluation indicated that our developed neural scaffold achieved satisfactory regenerative outcomes, which were very close to those achieved by autologous nerve grafts, the accepted golden standard for peripheral nerve repair. Moreover, additional therapeutic benefits produced by the modification of a neural scaffold with BMSC-derived ACM may be associated with the unique neural activity of the ACM, as evidenced by in vitro experimental findings that the ACM significantly enhanced axonal regrowth and Schwann cell proliferation. Our results will provide a further experimental basis for the translation of ACM-containing neural scaffolds into the clinic.
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