期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 5, 期 18, 页码 3377-3382出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6tb03345h
关键词
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资金
- National Natural Science Foundation of China [21607053, 21107029, 21275018]
- Shandong Province Key Research and Development Project [2016GSF117010]
- Postdoctoral Science Foundation of China [2013M541953, 2016M592127]
- Outstanding Young Scientists Award Fund of Shandong Province [BS2013HZ007]
- Shandong Provincial Natural Science Foundation [ZR2014DM011]
- Shandong Provincial Key Research Project [2015GSF117025]
- Beijing National Laboratory for Molecular Sciences (BNLMS) [20140121]
- Doctoral Program of Higher Education of China (RFDP) [20121101110049]
- 111 Project [B07012]
The development of techniques for detecting HOCl at the subcellular level is very important to elucidate its cellular functions. Due to its relatively low concentration, it is still a great challenge to specifically track the basal HOCl in normal cells. In this paper, based on the unique chlorination of HOCl by the initiation of chlorinium ions (Cl+) in an acidic medium, we have developed a simple pH-mediated lysosome-targetable fluorescent probe Lyso-HOCl for the specific detection of HOCl over other bioactive molecules at higher concentration (500 mu M). Our results show that Lyso-HOCl possesses a detection limit of 8.0 pM, and can quantitatively detect HOCl at the picomolar level. The ultrasensitive and ultrafast response property of probe Lyso-HOCl offers a good opportunity to monitor the basal HOCl and the fluctuation of endogenous HOCl levels in the lysosomes of macrophages (Raw 264.7 cells), and we thus anticipate that this probe would provide a promising tool for further unraveling the biological functions of HOCl in subcellular lysosomes.
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