期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 5, 期 35, 页码 7262-7266出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7tb01394a
关键词
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资金
- i-sense EPSRC IRC in Early Warning Sensing Systems for Infectious Diseases [EP/K031953/1]
- Royal Society Wolfson Research Merit Award
- Biochemical Engineering Doctoral Training Award
- Engineering and Physical Sciences Research Council [EP/K031953/1] Funding Source: researchfish
- EPSRC [EP/K031953/1] Funding Source: UKRI
We report the development of a tuneable plasmonic nanochain immunoassay with increased sensitivity over traditional monodisperse nanoparticle lateral flow tests. Our approach takes advantage of the unique self-assembling properties of polyamidoamine dendrimers with gold nanoparticles in aqueous media to create one-dimensional nanochains, with a distinct red to blue colour change, attributable to a longitudinal plasmon resonance, which can be readily detected by eye and a digital camera. We optimise and characterise nanochain formation and stability using UV-visible spectroscopy, transmission electron microscopy and dynamic light scattering. As a proof-of-principle we focus on the application of nanochains for point-of-care diagnostics for p24, an important biomarker of early HIV infections and successfully detect p24 with a limit of detection of 5 ng ml(-1) in pseudo-serum, 4 fold more sensitive than comparable studies with gold nanoparticles. These findings and underlying concepts highlight the potential of advanced functional organic-inorganic composite nanomaterials to diagnose infections, with broad applicability to non-communicable diseases.
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