期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 149, 期 -, 页码 288-296出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.10.032
关键词
Chitosan; Dextran sulfate; Nanoparticles; Cornea; Microfluorometer
资金
- Thailand Research Fund (TRF) under Royal Golden Jubilee Ph. D. Program [PHD/0094/2554]
- Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on High Education, Ministry of Education, Thailand
- Naresuan University
- Agricultural Research Development Agency, Thailand
Topical application of drugs to the eyes suffers from poor bioavailability at the ocular surface and in the anterior chamber. This is due to rapid clearance of the drug because of tear secretion and outflow. This study has investigated mucoadhesive and penetration characteristics of chitosan-dextran sulfate nanoparticles (CDNs), prepared by polyelectrolyte complexation technique, following topical administration to the ocular surface. Topical FITC-labeled CDNs (FCDNs; mean size of 400 nm and a surface charge of +48 mV) were retained on the porcine ocular surface for more than 4 h. Topical FCDNs were partially endocytosed into porcine corneal epithelial cells via a clathrin-dependent pathway. After 6 h of topical FCDNs, particles accumulated in the corneal epithelium but not found in the corneal stroma. When epithelium was removed, FCDNs penetrated the stroma. Thus, CDNs are potentially useful for drug/gene delivery to the ocular surface and to stroma when epithelium is damaged. (C) 2016 Elsevier B.V. All rights reserved.
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