期刊
BIOTECHNOLOGY ADVANCES
卷 35, 期 1, 页码 64-76出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2016.11.008
关键词
Cell engineering; Chinese hamster ovary (CHO) cells; Ectopic expression; Endoplasmic reticulum; ER stress; Gene dosage; Product quality; Recombinant protein production; Secretion bottleneck; Specific productivity
资金
- Novo Nordisk Foundation
- European Union [642663]
- NNF Center for Biosustainability [CHO Cell Line Engineering & Design] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
- Marie Curie Actions (MSCA) [642663] Funding Source: Marie Curie Actions (MSCA)
Chinese hamster ovary (CHO) cells are the preferred cell factory for the production of therapeutic glycoproteins. Although efforts primarily within bioprocess optimization have led to increased product titers of recombinant proteins (r-proteins) expressed in CHO cells, post-transcriptional bottlenecks in the biosynthetic pathway of r-proteins remain to be solved. To this end, the ectopic expression of transgenes (effector genes) offers great engineering potential. However, studies on effector genes have in some cases led to inconsistent results. Whereas this can in part be attributed to product specificity, other experimental and cellular factors are likely important contributors to these conflicting results. Here, these factors are reviewed and discussed with the objective of guiding future studies on effector genes. (C) 2016 Elsevier Inc. All rights reserved.
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