期刊
JOURNAL OF DIABETES RESEARCH
卷 2017, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2017/4802319
关键词
-
资金
- National Natural Science Foundation of China [81600695]
- Natural Science Foundation of Shandong Province [ZR2014HM042, ZR2015HM031, ZR2015PH031]
- Promotive Research Fund for Excellent Young and Middle-Aged Scientists of Shandong Province [BS2015YY007]
- Medicine and Health Science Technology Development Projects of Shandong Province [2014WS0237, 2016WS0744, 2016WS0747]
Background. Tumor necrosis factor-a-induced protein 8-like 2 (TIPE2 or TNFAIP8L2) is a negative regulator of natural and adaptive immunity. The role of TIPE2 in type 2 diabetes mellitus (T2DM) remains unknown, although TIPE2 plays key roles in preserving inflammatory homeostasis. Methods. TIPE2 expression was measured by Western blotting and real-time polymerase chain reaction (RT-PCR) in peripheral blood mononuclear cells (PBMCs) isolated from T2DM patients and healthy controls, and tumor necrosis factor-alpha (TNF-alpha), high-sensitivity C-reactive protein (hsCRP), interleukin 6 (IL-6), and other related biometabolic parameters were detected using a nephelometer or by ELISA. Differentiated THP-1 cells were exposed to siTIPE2 and TIPE2 adenovirus. Results. TIPE2 was significantly increased in PBMCs from T2DM patients compared with those from healthy controls and was negatively correlated with serum TNF-alpha, IL-6, and hsCRP concentrations but positively correlated with HbA1c and LDL-C in T2DM patients. High glucose treatment (50 mmol/L) can upregulate the expression of TIPE2 and cytokine secretion in differentiated THP-1 cells. siTIPE2 infection exacerbated the increased TNF-alpha and IL-6 concentrations in differentiated THP-1 cells under high glucose conditions (50 mmol/L), while infection with TIPE2 adenovirus reversed the increased TNF-alpha concentration. Conclusions. The present study indicates that TIPE2 may participate in T2DM by regulating TNF-alpha production.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据