期刊
CELL DEATH DISCOVERY
卷 3, 期 -, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/cddiscovery.2016.97
关键词
-
类别
资金
- National Key Research Program of China [2016YFA0100903]
- National Funds for Distinguished Young Scientists of China [81325009]
- National Nature Science Foundation of China [81270168, 81227901, 81530058, BWS12J037]
- Beijing Nature Science Foundation [7152131]
- Innovation Team Grant of Shanxi Province
Sepsis-induced cardiac dysfunction remains a major cause of morbidity and mortality in patients suffered from severe trauma. Mesenchymal stem cells (MSCs) -based treatment has been verified as a promising approach to mitigate the sepsis-induced cardiac dysfunction, but the mechanism is still ambiguous. Thus, our study was designed to explore the potential role of MSCs in sepsis-induced cardiac dysfunction. In vivo bioluminescence imaging revealed 80% acute donor cell death of bone marrow-derived MSCs (BM-MSCs) within 3 days after transplantation. However, echocardiography demonstrated that systolic function in wild-type mice group were reduced after sepsis, while the cardiac function was relatively well persevered in cardiac-conditional deletion of Raptor (component of mTORC1 complex) mice group. Raptor KO group treated with BM-MSCs appeared better cardiac function than other groups (P < 0.05). In vitro cell study revealed that co-culture of H9C2 (Raptor-Knock down) and BM-MSC could attenuate the level of proinflammatory cytokines and promote the expression of anti-inflammatory cytoldne accompanied by mTORC2-Akt activation (P < 0.05). In contrast, co-culture H9C2 (Raptor-O.E) and BM-MSC could aggravate the inflammatory response accompanied by the activation of mTORC1-p7056K and inhibition of mTORC2-Akt (P < 0.05). The immunomodulatory property of MSC is related to the inhibition of mTORC1-p70S6K and activation of mTORC2-Akt signaling pathway. mTORC1-p7056K and mTORC2-Ala pathways were involved in the therapeutic adjuncts of MSC. The possible mechanism due to MSC's immunomodulatory property through activation of mTORC2-Akt and inhibition of mTORC1-p70S6K signal pathways which may lead to modulate the expression of inflammation cytokines.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据