期刊
JOURNAL OF NUCLEIC ACIDS
卷 2017, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2017/6513720
关键词
-
资金
- Slovak Research and Development Agency [APVV-0280-11]
- European Cooperation in Science and Technology [COST CM1406]
- Slovak Grant Agency [1/0131/16]
- internal university grant [VVGS-PF-2017-251, VVGS-2016-2596]
The HIV virus is one of the most studied viruses in the world. This is especially true in terms of gene sequencing, and to date more than 9 thousand genomic sequences of HIV isolates have been sequenced and analyzed. In this study, a series of DNA sequences, which have the potential to form G-quadruplex structures, is analyzed. Several such sequences were found in various coding and noncoding virus domains, including the U3LTR, tat, rev, env, and vpx regions. Interestingly, a homological sequence to the already well-known HIV integrase aptamer was identified in the minus-strand. The sequences derived from original isolates were analyzed using standard spectral and electrophoretic methods. In addition, a recently developed methodology is applied which uses induced circular dichroism spectral profiles of G-quadruplex-ligand (Thiazole Orange) complexes to determine if G-rich sequences can adopt G-quadruplex structure. Targeting the G-quadruplexes or peptide domains corresponding to the G-rich coding sequence in HIV offers researchers attractive therapeutic targets which would be of particular use in the development of novel antiviral therapies. The analysis of G-rich regions can provide researchers with a path to find specific targets which could be of interest for specific types of virus.
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