期刊
CHEMICAL COMMUNICATIONS
卷 53, 期 2, 页码 424-427出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc08542c
关键词
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资金
- Foundation of the Thousand Talents Plan for Young Professionals and Jiangsu Specially-Appointed Professor
- Yangzhou University, Strategic Priority Research Program of the Chinese Academy of Sciences [XDA09030306]
- National Natural Science Foundation of China [31530026]
- Key Research Program of Frontier Sciences, CAS [QYZDB-SSW-SMC013]
- Beijing Natural Science Foundation [5164037]
- China Postdoctoral Science Foundation [2015M570158]
- China Postdoctoral Science Special Foundation [2016T90143]
The Fe3O4 nanozyme was the first reported nanoparticle with intrinsic peroxidase-like activity and has been widely used in biomedicine. To optimize its catalytic activity, we introduced histidine residues onto the Fe3O4 nanoparticle surface in order to mimic the enzymatic microenvironment of natural peroxidase enzymes. Our results show that modification with a single amino acid could more than ten-fold improve the apparent affinity (KM) of the Fe3O4 nanozyme for the substrate H2O2 and enhanced its catalytic efficiency (k(cat)/K-M) up to twenty fold. Thus we not only optimized the activity of the Fe3O4 nanozyme, but also provide a new rationale for improving the efficiency of nanomaterial-based catalysts by utilizing strategies observed in nature.
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