期刊
BIOSENSORS & BIOELECTRONICS
卷 87, 期 -, 页码 679-685出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.08.087
关键词
Resonance Light Scattering (RLS) sensor; Virus-molecularly imprinted polymer (virus-MIPs); Hepatitis A Virus (HAV)
类别
资金
- National Natural Science Foundation of China [21305118, 21402168, 21505112]
- Science and Technology Department of Hunan Province [2013SK2021]
- Scientific Research Foundation of Hunan Provincial Education Department [15B232, 16A204]
We described a novel resonance light scattering (RLS) sensor for the specific recognition of trace quantities of Hepatitis A Virus (HAV); the sensor was based on a mussel-inspired hepatitis molecularly imprinted polymer. As a recognition element, polydopamine (PDA)-coated totivirus-imprinted polymer was introduced on the surface of SiO2 nanoparticles (virus-imprinted SiO2@PDA NPs) using an efficient one-step synthesis method. The target virus was selectively captured by the imprinted polymer films, thereby increasing the RLS intensity. A simple fluorescence spectrophotometer was employed.to measure the changes in the intensity. The enhanced RLS intensity (Delta I-RLS) was proportional to the concentration of HAV in the range of 0.04-6.0 nmol.L-1, with a low limit of detection of 8.6 pmol.L-1. The selectivity study confirmed that the resultant HAV-imprinted SiO2@PDA NPs possessed high selectivity for HAV. The sensor was successfully applied for the direct detection of additional HAV from a 20,000-fold dilution of human serum. The proposed strategy is simple, eco-friendly, highly selective, and sensitive. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据