期刊
BIOSENSORS & BIOELECTRONICS
卷 87, 期 -, 页码 59-65出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.07.097
关键词
Surface-enhanced Raman spectroscopy (SERS); Optical sensor; Mercury ions; Oligonucleotide; Sliver nanorods array
类别
资金
- National Key Basic Research Program of China (973) [2012CB933301]
- National Natural Science Foundation of China [61302027]
- Natural Science Foundation of Jiangsu Province of China [BK20130871]
- Sci-tech Support Plan of Jiangsu Province [BE2014719]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R37]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [YX03001]
- Suzhou International Sci. & Tech. Cooperation Program [SH201111]
With years of outrageous mercury emissions, there is an urgent need to develop convenient and sensitive methods for detecting mercury ions in response to increasingly serious mercury pollution in water. In the present work, a portable, ultrasensitive SERS sensor is proposed and utilized for detecting trace mercury ions in water. The SERS sensor is prepared on an excellent sliver nanorods array SERS substrate by immobilizing T-component oligonucleotide probes labeled with dye on the 3'-end and -SH on the 5'-end. The SERS sensor responses to the specific chemical bonding between thymine and mercury ions, which causes the previous flexible single strand of oligonucleotide probe changing into rigid and upright double chain structure. Such change in the structure drives the dyes far away from the excellent SERS substrate and results in a SERS signal attenuation of the dye. Therefore, by monitoring the decay of SERS signal of the dye, mercury ions in water can be detected qualitatively and quantitatively. The experimental results indicate that the proposed optimal SERS sensor owns a linear response with wide detecting range from 1 pM to 1 mu M, and a detection limit of 0.16 pM is obtained. In addition, the SERS sensor demonstrates good specificity for Hg2+, which can accurately identify trace mercury ions from a mixture of ten kinds of other ions. The SERS sensor has been further executed to analyze the trace mercury ions in tap water and lake water respectively, and good recovery rates are obtained for sensing both kinds of water. With its high selectivity and good portability, the ultrasensitive SERS sensor is expected to be a promising candidate for discriminating mercury ions in the fields of environmental monitoring and food safety. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据