期刊
CARBOHYDRATE POLYMERS
卷 156, 期 -, 页码 165-174出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.09.029
关键词
Microwave-assisted extraction; Pressurized liquid extraction; Response surface methodology; beta-D-Glucans; P. ostreatus; G. lucidum
资金
- Brazilian funding agency CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- Brazilian funding agency CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- Brazilian funding agency Fundacao Araucaria
- Spanish MINECO (Ministerio de Economia y Competitividad) [JCI-2012-12972]
- national R + D program from the Spanish Ministry of Science and Innovation [AGL2014-56211-R]
- regional program from the Community of Madrid, Spain [S2013/ABI-2728]
Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active (beta-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies. Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent. Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors. Temperature showed stronger influence in the polysaccharide extraction than time. The latter factor slightly affected MAE but not PLE extractions. Optimal conditions within the studied range were determined for each extraction method and species based on the desirability functions. Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly beta- and alpha-glucans and heteropolysaccharides. Both extraction systems were effective for polysaccharide extraction from mushrooms. (C) 2016 Elsevier Ltd. All rights reserved.
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