期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 3, 页码 2052-2058出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b12047
关键词
silicon quantum dots; chlorin e6 (Ce6); ratiometric fluorescence probe; highly reactive oxygen species; hydroxyl radical ((OH)-O-center dot); cell imaging
资金
- National Natural Science Foundation of China [21375027, 21335002, 21427806]
- Shanghai Nature Science Foundation [12ZR1401700]
The monitoring of reactive oxygen species (ROS) in living cells remains challenging because of the complexity, short half-life, and autofluorescence of biological samples. In this work, we designed a ratiometric fluorescent probe for the detection and imaging of ROS, which was constructed from silicon quantum dots (Si QDs) with chlorin e6 (Ce6) through electrostatic attraction and showed well-resolved dual fluorescence emission signals (490 and 660 nm). Sensitive and selective biosensing of hydroxyl radical ((OH)-O-center dot) was demonstrated on the basis of fluorescence quenching of the Si QDs and Ce6 as an internal reference to avoid environmental interference, with a detection limit of similar to 0.97 mu M. The endogenous release of (OH)-O-center dot was also monitored and imaged in living cells.
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