期刊
HAEMATOLOGICA
卷 102, 期 9, 页码 1594-1604出版社
FERRATA STORTI FOUNDATION
DOI: 10.3324/haematol.2016.163337
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资金
- Televie grant by F.R.S.-FNRS (Fonds National de la Recherche Scientifique) of Belgium
- David and Alice Van Buuren Fund''
- Fonds IRIS-Recherche''
- Les Amis de l'Institut Jules Bordet
- FER (Fonds d'Encouragement a la Recherche) of the Faculty of Medicine
- Universite Libre de Bruxelles
- foundation Lambeau-Marteaux
- European Regional Development Fund
- Wallonia
- projet de recherche (PDR) by the F.R.S.-FNRS (Fonds National de la Recherche Scientifique) of Belgium
Interactions between chronic lymphocytic leukemia (CLL) B cells and the bone marrow (BM) microenvironment play a major function in the physiopathology of CLL. Extracellular vesicles (EVs), which are composed of exosomes and microparticles, play an important role in cell communication. However, little is known about their role in CLL / microenvironment interactions. In the present study, EVs purified by ultracentrifugation from BM mesenchymal stromal cell (BM- MSC) cultures were added to CLL B cells. After their integration into CLL B cells, we observed a decrease of leukemic cell spontaneous apoptosis and an increase in their chemoresistance to several drugs, including fludarabine, ibrutinib, idelalisib and venetoclax after 24 hours. Spontaneous (P= 0.0078) and stromal cell-derived factor 1a-induced migration capacities of CLL B cells were also enhanced (P= 0.0020). A microarray study highlighted 805 differentially expressed genes between leukemic cells cultured with or without EVs. Of these, genes involved in the B-cell receptor pathway such as CCL3/4, EGR1/2/3, and MYC were increased. Interestingly, this signature presents important overlaps with other microenvironment stimuli such as B-cell receptor stimulation, CLL/nurse-like cells co-culture or those provided by a lymph node microenvironment. Finally, we showed that EVs from MSCs of leukemic patients also rescue leukemic cells from spontaneous or drug-induced apoptosis. However, they induce a higher migration and also a stronger gene modification compared to EVs of healthy MSCs. In conclusion, we show that EVs play a crucial role in CLL B cells/BM microenvironment communication.
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