期刊
GENETICS
卷 206, 期 3, 页码 1611-1619出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.115.186205
关键词
allelic diversity; genetic load; haploidy linkage disequilibrium; maize
资金
- German Ministry of Education and Research (BMBF) within the AgroClustEr Synbreed-Synergistic plant and animal breeding [FKZ: 0315562D]
- project MAZE: Accessing the genomic and functional diversity of maize to improve quantitative traits [FKZ: 031B0195F]
Thousands of landraces are stored in seed banks as gold reserves for future use in plant breeding. In many crops, their utilization is hampered because they represent heterogeneous populations of heterozygous genotypes, which harbor a high genetic load. We show, with high-density genotyping in five landraces of maize, that libraries of doubled-haploid (DH) lines capture the allelic diversity of genetic resources in an unbiased way. By comparing allelic differentiation between heterozygous plants from the original landraces and 266 derived DH lines, we find conclusive evidence that, in the DH production process, sampling of alleles is random across the entire allele frequency spectrum, and purging of landraces from their genetic load does not act on specific genomic regions. Based on overall process efficiency, we show that generating DH lines is feasible for genetic material that has never been selected for inbreeding tolerance. We conclude that libraries of DH lines will make genetic resources accessible to crop improvement by linking molecular inventories of seed banks with meaningful phenotypes.
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