期刊
ANALYTICA CHIMICA ACTA
卷 952, 期 -, 页码 81-87出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.11.041
关键词
Zn-doped magnetic nanoclusters; Direct immobilization of antibodies; Sensitive and selective pathogenic bacteria detection
资金
- National Research Foundation of Korea (NRF) - Korea government (MEST) [NRF-2014R1A2A2A01007027]
Zinc-doped magnetic nanoclusters (Zn-MNCs) were synthesized and used to detect pathogenic bacteria in milk. Hydrothermally synthesized Zn-MNCs exhibited stronger magnetic properties than pure MNCs, which facilitated the magnetic separation from the sample using a permanent magnet. The presence of accessible Zn sites allows the direct immobilization of half-fragmented antibodies over Zn-MNCs through strong Zn-S bonds and prevents the tedious multiple steps of molecular functionalization or coating with costly noble metals prior to conjugation with an antibody. After the capture and magnetic separation of Salmonella in milk using the antibody-functionalized Zn-MNCs, the concentration of bacteria was determined with a portable ATP luminometer and the detection limit was found to be 10 CFU/mL. (C) 2016 Elsevier B.V. All rights reserved.
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