期刊
FRONTIERS IN PLANT SCIENCE
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2017.01337
关键词
Suaeda salsa; glycerol-3-phosphate acyltransferase; salt stress; PG; unsaturated fatty acids; seedling
资金
- NSFC (National Natural Science Research Foundation of China) [31300205]
- Natural Science Research Foundation of Shandong [ZR2016JL028, ZR2013CQ009]
- Science and Technology Development Projects of Shandong Province [2014GNC113005]
- Opening Foundation of the State Key Laboratory of Crop Biology, China [2015KF01]
- Opening Foundation of Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology
- Program For Scientific research innovation team in colleges and universities of Shandong Province
Glycerol-3-phosphate acyltransferase is the first acyl esterifying enzyme in phosphatidylglycerol (PG) synthesis process. In this study, we isolated and characterized the glycerol-3-phosphate acyltransferase (GPAT) gene from Suaeda salsa (S. salsa) and obtained the full length of the GPAT gene from S. salsa (SsGPAT) by 5 ' and 3 ' RACE. The clone contained an open reading frame (ORF) of 1167 bp nucleotides that comprised of 388 amino acid residues. Real-time PCR revealed that the mRNA accumulation of GPAT in S. salsa was induced by salt stress. The highest expression levels were observed when S. salsa leaves were exposed to 300 mM NaCl treatment. At the germination stage, the germination rate and root length of overexpressed Arabidopsis strains were significantly higher than WT under different concentrations of NaCl treatments, while the inhibitory effect was significantly severe in T-DNA insertion mutant strains. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (Delta I/Io), and the unsaturated fatty acid content of PG decreased less in overexpressed strains and more in mutant strains than that in WT under salt stress. These results suggest that the overexpression of SsGPAT in Arabidopsis enhances salt tolerance and alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of PG.
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