期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 87, 期 2, 页码 95-99出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2016.03.011
关键词
Shiga toxin; Shiga toxigenic Escherichia coli; Escherichia coli; Immunoassay; Rapid
资金
- Alere(TM)
In this study we evaluated the performance of the SHIGA TOXIN QUIK CHEK (Techlab (R), Blacksburg, VA) and the ImmunoCard STAT! Enterohaemorrhagic E. coil (EHEC) (Meridian BioScience, Cincinnati, OH, USA) assays as methods for qualitatively detecting the presence of Shiga toxin in human fecal specimens. A multiplex PCR for the detection of stx1 and stx2 was used as the standard for comparison. The SHIGA TOXIN QUIK CHEK detected all known Shiga toxin subtypes with the exception of Stx2f, while the ImmunoCard STAT! EHEC was unable to identify four of the seven Stx2 subtypes, including Stx2b and Stx1d. When compared to multiplex PCR based on Shiga toxin gene presence alone both assays demonstrated 100% specificity, and gave sensitivity values of 50.0% and 41.2% respectively. Correlation between each assay and the multiplex PCR was calculated by the use of kappa, with both assays exhibiting a moderate level of agreement. Crown Copyright (C) 2016 Published by Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据