期刊
BMC IMMUNOLOGY
卷 18, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12865-016-0185-5
关键词
Signaling pathway; MAPK; NF-kappa B; Oral cells; P. gingivalis
类别
资金
- von-Behring-Rontgen-Stiftung grant
Background: The bacterial biofilm at the gingival margin induces a host immune reaction. In this local inflammation epithelial cells defend the host against bacterial challenge. Porphyromonas gingivalis (P. gingivalis), a keystone pathogen, infects epithelial cells. The aim of this study was to investigate the activation of signaling cascades in primary epithelial cells and oral cancer cell lines by a profiler PCR array. Results: After infection with P. gingivalis membranes the RNA of 16 to 33 of 84 key genes involved in the antibacterial immune response was up-regulated, amongst them were IKBKB (NF-kappa B signaling pathway), IRF5 (TLR signaling) and JUN, MAP2K4, MAPK14 and MAPK8 (MAPK pathway) in SCC-25 cells and IKBKB, IRF5, JUN, MAP2K4, MAPK14 and MAPK8 in PHGK. Statistically significant up-regulation of IKBKB (4.7 x), MAP2K4 (4.6 x), MAPK14 (4.2 x) and IRF5 (9.8 x) (p < 0.01) was demonstrated in SCC-25 cells and IKBKB (3.1 x), MAP2K4 (4.0 x) MAPK 14 (3.0 x) (p < 0.05), IRF5 (3.0 x) and JUN (7. 7 x) (p < 0.01) were up-regulated in PHGK. Conclusions: P. gingivalis membrane up-regulates the expression of genes involved in downstream TLR, NF kappa B and MAPK signaling pathways involved in the pro-inflammatory immune response in primary and malignant oral epithelial cells.
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