期刊
FRONTIERS IN MICROBIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2017.00088
关键词
autotrophy; CO2 fixation; stable C isotopes; geothermal; Aquificales; Crenarchaeota
类别
资金
- DOE-Pacific Northwest National Laboratory [112443, 254840]
- Department of Energy (DOE)-Joint Genome Institute Community Sequencing Program [CSP 787081, CSP701]
- National Science Foundation IGERT Program [NSF DGE 0654336]
- Genomic Science Program, Office of Biological and Environmental Research, U.S. DOE [DOE-AC02-05CH11231]
Although the biological fixation of CO2 by chemolithoautotrophs provides a diverse suite of organic compounds utilized by chemoorganoheterotrophs as a carbon and energy source, the relative amounts of autotrophic C in chemotrophic microbial communities are not well-established. The extent and mechanisms of CO2 fixation were evaluated across a comprehensive set of high-temperature, chemotrophic microbial communities in Yellowstone National Park by combining metagenomic and stable (13)G isotope analyses. Fifteen geothermal sites representing three distinct habitat types (iron-oxide mats, anoxic sulfur sediments, and filamentous streamer communities) were investigated. Genes of the 3-hydroxypropionate/4-hydroxybutyrate, dicarboxylate/4-hydroxybutyrate, and reverse tricarboxylic acid CO2 fixation pathways were identified in assembled genome sequence corresponding to the predominant Crenarchaeota and Aquificales observed across this habitat range. Stable C-13 analyses of dissolved inorganic and organic C (DIG, DOC), and possible landscape C sources were used to interpret the C-13 content of microbial community samples. Isotope mixing models showed that the minimum fractions of autotrophic C in microbial biomass were->50% in the majority of communities analyzed. The significance of CO2 as a C source in these communities provides a foundation for understanding community assembly and succession, and metabolic linkages among early-branching thermophilic autotrophs and heterotrophs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据