期刊
BRAIN BEHAVIOR AND IMMUNITY
卷 60, 期 -, 页码 270-281出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2016.11.005
关键词
Microglia; Middle cerebral artery occlusion; Neonate; Cytokine; AlphaV beta3 integrin
资金
- Fundacion Ramon Areces, Madrid, Spain
- NSFC [31260242]
- [RO1 NS44025]
- [RO1 NS76726]
The pathophysiology of neonatal stroke and adult stroke are distinct in many aspects, including the inflammatory response. We previously showed endogenously protective functions of microglial cells in acute neonatal stroke. We asked if galectin-3 (Gal3), a pleotropic molecule that mediates interactions between microglia/macrophages and the extracellular matrix (ECM), plays a role in early injury after transient middle cerebral occlusion (tMCAO) in postnatal day 9-10 mice. Compared to wild type (WT) pups, in Gal3 knockout pups injury was worse and cytokine/chemokine production altered, including further increase of MIP1 alpha. and MIP1 beta levels and reduced IL6 levels 72 h after tMCAO. Lack of Gal3 did not affect morphological transformation or proliferation of microglia but markedly attenuated accumulation of CD11b(+)/CD45(med-high) cells after injury, as determined by multi-color flow cytometry. tMCAO increased expression of alpha V and beta(3) integrin subunits in CD11b(+)/CD45(low) microglial cells and cells of non-monocyte lineage (CD11b(-)/CD45(-)), but not in CD11b(+)/CD45(med-high) cells within injured regions of WT mice or Gal3-/- mice. alpha V upregulated in areas occupied and not occupied by CD68(+) cells, most prominently in the ECM, lining blood vessels, with expanded alpha V coverage in Gal3-/- mice. Cumulatively, these data show that lack of Gal3 worsens subchronic injury after neonatal focal stroke, likely by altering the neuroinflammatory milieu, including an imbalance between pro- and anti-inflammatory molecules, effects on microglial activation, and deregulation of the composition of the ECM. (C) 2016 Elsevier Inc. All rights reserved.
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