期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 8, 页码 2026-2029出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201611005
关键词
biosynthesis; enzymes; natural products; peptidomimetics; pseudopeptides
资金
- MEXT, Japan (JSPS KAKENHI) [23108101, 16H06452]
- JSPS [25560397, 15H03110, 16K18692]
- Grants-in-Aid for Scientific Research [25560397, 15H03110, 16K18692] Funding Source: KAKEN
We recently discovered novel pseudotripeptides, the ketomemicins, which possess a C-terminal pseudodipeptide connected with a carbonylmethylene instead of an amide bond, through heterologous expression of gene clusters identified in actinobacteria. The carbonylmethylene structure is a stable isostere of the amide bond and its biological significance has been shown in several natural and synthetic products. Despite the biological importance of these compounds, little is known about how the carbonylmethylene structure is biosynthesized. In this work, we fully characterized the biosynthetic machinery of the pseudodipeptide. An aldolase, dehydratase, PLP-dependent glycine-C-acetyltransferase, and dehydrogenase were involved in the formation of the pseudodipeptide, with malonyl-CoA and phenylpyruvate as starter substrates.
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