期刊
APPLIED ENERGY
卷 188, 期 -, 页码 392-398出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.apenergy.2016.12.044
关键词
Laccase-catalyzed cathode; White-rot fungi; Electricity generation; Azo dye; Decolorization
资金
- Ministry of Science and Technology of the Republic of China, Taiwan [MOST 105-2221-E-224-003]
Wood-degrading white-rot fungi produce many extracellular enzymes, including the multi-copper oxidative enzyme laccase (EC 1.10.3.2). Laccase uses atmospheric oxygen as the electron acceptor to catalyze a one-electron oxidation reaction of phenolic compounds and therefore has the potential to simultaneously act as a cathode catalyst in a microbial fuel cell (MFC) and degrade azo dye pollutants. In this study, the laccase-producing white-rot fungus Ganoderma lucidum BCRC 36123 was planted on the cathode surface of a single-chamber MFC to degrade the azo dye acid orange 7 (AO7) synergistically with an anaerobic microbial community in the anode chamber. In a batch culture, the fungus used AO7 as the sole carbon source and produced laccase continuously, reaching a maximum activity of 20.3 +/- 0.3 U/L on day 19 with a 77% decolorization of the dye (50 mg/L). During MFC operations, AO7 in the anolyte diffused across a layer of polyvinyl alcohol-hydrogel that separated the cathode membrane from the anode chamber, and served as a carbon source to support the growth of, and production of laccase by, the fungal mycelium that was planted on the cathode. In such MFCs, laccase-producing fungal cathodes outperformed laccase-free controls, yielding a maximum open-circuit voltage of 821 mV, a closed-circuit voltage of 394 mV with an external resistance of 1000 Omega, a maximum power density of 13.38 mW/m(2), a maximum current density of 33 mA/m(2), and a >90% decolorization of AO7. This study demonstrates the feasibility of
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