期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 6, 页码 5077-5091出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b13576
关键词
self-assembled; 3D nickel nanonetwork; SERS activation; nano biosensor; glutathione; crystal violet; L-cysteine; L-methionine
资金
- Natural Science and Engineering Research Council of Canada (NSERC Discovery Grant) [126042, 119087]
We introduce a non-noble metal based SERS active nanobiosensor using a self-assembled 3D hybrid nickel nanonetwork. A tunable biomolecule detector fabricated by a bottom-up approach was functionalized using a multiphoton ionization energy mechanism to create a self-assembled 3D hybrid nickel nanonetwork. The nanonetwork was tested for SERS detection of crystal violet (CV) and glutathione (GSH) at two excitation wavelengths, 532 and 785 nm. The results reveal indiscernible peaks with a limit of detection (LOD) of 1 picomolar (pM) concentration. An enhancement factor (EF) of 9.3 x 10(8) was achieved for the chemical molecule CV and 1.8 x 10(9) for the biomolecule GSH, which are the highest reported values so far. The two results, one being the CV molecule proved that nickel nanonetwork is indeed SERS active and the second being the GSH biomolecule detection at both 532 and 785 nm, confirm that the nanonetwork is a biosensor which has potential for both in vivo and in vitro sensing. In addition, the selectivity and versatility of this biosensor is examined with biomolecules such as L-Cysteine, L-Methionine, and sensing GSH in cell culture medium which mimics the complex biological environment. The functionalized self-assembled 3D hybrid nickel nanonetwork exhibits electromagnetic and charge transfer based SERS activation mechanisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据