期刊
DEVELOPMENTAL CELL
卷 40, 期 4, 页码 331-341出版社
CELL PRESS
DOI: 10.1016/j.devcel.2017.01.015
关键词
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资金
- EMBL Advanced Light Microscopy, Protein Core Facility
- Laboratory Animal Resource facilities
- EMBL Interdisciplinary Postdoc Program under the Marie Curie COFUND Actions [18877]
- Cluster of Excellence Cellular Networks - CellNetworks grant (Metabolism and Development), University of Heidelberg [EcTOP6]
How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from C-13-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation.
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