期刊
ELIFE
卷 6, 期 -, 页码 -出版社
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.21763
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资金
- Natural Sciences and Engineering Research Council of Canada [435521-2013]
- Canada Research Chairs [950-228994]
- Canadian Institutes of Health Research [142473]
- Canada Foundation for Innovation [228994]
- Wellcome Junior Research Fellowship
- Biotechnology and Biological Sciences Research Council BBSRC [BB/N006453/1]
- Medical Research Council MRC [MR/K01580X/1]
- BBSRC [BB/P000746/1, BB/N006453/1] Funding Source: UKRI
The replisome is a multiprotein machine that carries out DNA replication. In Escherichia coli, a single pair of replisomes is responsible for duplicating the entire 4.6 Mbp circular chromosome. In vitro studies of reconstituted E. coli replisomes have attributed this remarkable processivity to the high stability of the replisome once assembled on DNA. By examining replisomes in live E. coli with fluorescence microscopy, we found that the Pol III* subassembly frequently disengages from the replisome during DNA synthesis and exchanges with free copies from solution. In contrast, the DnaB helicase associates stably with the replication fork, providing the molecular basis for how the E. coli replisome can maintain high processivity and yet possess the flexibility to bypass obstructions in template DNA. Our data challenges the widely-accepted semidiscontinuous model of chromosomal replication, instead supporting a fully discontinuous mechanism in which synthesis of both leading and lagging strands is frequently interrupted.
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