期刊
CARBOHYDRATE POLYMERS
卷 159, 期 -, 页码 195-206出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.12.013
关键词
Monostroma angicava; Sulfated polysaccharide; Structure; Anticoagulant activity; Inhibition mechanism
资金
- National Natural Science Foundation of China [41476108]
- Science and Technology Development Program of Shandong Province, China [2014GHY115015]
- NSFC-Shandong Joint Fund for Marine Science Research Centers [U1406402]
- Development Program of Blue Biological Resource [2015ASKJ02]
An anticoagulant-active polysaccharide PF2 was extracted with boiling water from the green seaweed Monostroma angicava, further purified by anion-exchange and size-exclusion chromatography. PF2 was a rhamnan-type sulfated polysaccharide with molecular weight of about 88.1 kDa. Results of chemical and spectroscopic analyses demonstrated that PF2 consisted of -> 43)-alpha-L-Rhap-(1 -> and -> 2)-alpha-L-Rhap-(1 -> residues, with partially branches at C-2 of -> 3)-alpha-L-Rhap-(1 -> residues. Sulfate groups were substituted at C-3 of -> 2)-alpha-L-Rhap-(1 -> residues. The sulfated polysaccharide PF2 had a high anticoagulant action, and the mechanism of anticoagulant activity mediated by PF2 was mainly attributed to strong potentiation thrombin by heparin cofactor II. PF2 also exhibited weak effect on antithrombin-dependent thrombin or factor Xa inhibition. The fibrin(ogen)olytic activity and thrombolytic activity of PF2 were also evaluated. The investigation revealed that PF2 was a novel sulfated rhamnan differing from previously described sulfated polysaccharides from green seaweed and could be a potential anticoagulant polysaccharide. (C) 2016 Elsevier Ltd. All rights reserved.
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