期刊
MICROORGANISMS
卷 5, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms5010007
关键词
Bacillus subtilis; biofilm; calcium; surfactin; sliding; colony expansion
类别
资金
- Marie Sklodowska Curie career integration grant (PheHetBacBiofilm)
- Deutsche Forschungsgemeinschaft (DFG) [KO4741/2-1, KO4741/3-1]
- Jena School for Microbial Communications (JSMC) fellowship
- International Max Planck Research School (IMPRS) fellowship
Robust colony formation by Bacillus subtilis is recognized as one of the sessile, multicellular lifestyles of this bacterium. Numerous pathways and genes are responsible for the architecturally complex colony structure development. Cells in the biofilm colony secrete extracellular polysaccharides (EPS) and protein components (TasA and the hydrophobin Bs1A) that hold them together and provide a protective hydrophobic shield. Cells also secrete surfactin with antimicrobial as well as surface tension reducing properties that aid cells to colonize the solid surface. Depending on the environmental conditions, these secreted components of the colony biofilm can also promote the flagellum-independent surface spreading of B. subtilis, called sliding. In this study, we emphasize the influence of Ca2+ in the medium on colony expansion of B. subtilis. Interestingly, the availability of Ca2+ has no major impact on the induction of complex colony morphology. However, in the absence of this divalent ion, peripheral cells of the colony expand radially at later stages of development, causing colony size to increase. We demonstrate that the secreted extracellular compounds, EPS, BslA, and surfactin facilitate colony expansion after biofilm maturation. We propose that Ca2+ hinders biofilm colony expansion by modifying the amphiphilic properties of surfactin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据