期刊
ACS NANO
卷 11, 期 3, 页码 2452-2458出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b07600
关键词
CRISPR/Cas9; gene editing; CRISPR/Cas9 engineering; nanoparticle; CRISPR delivery; genome engineering
类别
资金
- NIH [GM077173]
- NSF [CHE-1307021]
- UMass OTCV grant
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1307021] Funding Source: National Science Foundation
Genome editing through the delivery of CRISPR/Cas9-ribonucleoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here, we report a remarkably highly efficient (similar to 90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the coengineering of Cas9 protein and carrier nanoparticles. This construct provides effective (similar to 30%) gene editing efficiency and opens up opportunities in studying genome dynamics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据